The TRUPCR® EGFR PCR Kit is an in vitro diagnostic test intended for the qualitative detection of EGFR somatic mutations in genomic DNA extracted from fresh, frozen or formalin fixed paraffin-embedded (FFPE) tissue.
The TRUPCR®EGFR PCR kit is based on allele specific amplification and is achieved by ARMS PCR. Taq DNA polymerase is extremely effective at distinguishing between a match and a mismatch at the 3’-end of a PCR primer. The kit is designed to selectively amplify mutant specific sequences in samples that contain a mixture of wild-type and mutated DNA.
The most common mutations are found in exons 19 and 21. The detection is achieved using fluorescent probes labelled with FAM and HEX. The TRUPCR® EGFR PCR Kit is composed of 9 assays for the detection of the EGFR mutations and a reference control gene of the EGFR region without any known polymorphisms/ mutations.
G719S (c.2155 G>A), G719C (c.2155 G>T), G719A (c.2155 G>C)
T790M (c.2369 C>T), S768I (c.2303 G>T), C797S (c.2390G>C), C797S (c.2389T>A), V769_D770insASV (2307_2308insGCCAGCGTG), D770_N771insG (2310_2311insGGT), H773_V774insH (2319_2320insCAC)
L858R (c.2573 T>G), L861Q (c.2582 T>A)
E746_A750del (2235_2249del15), E746_A750del (2236_2250del15) L747_P753>S (2240_2257del18), L747_A750>P (2239_2248TTAAGAGAAG>C)
E746_S752>V (2237_2255>T), L747_T751del (2240_2254del15), L747_S752del (2239_2256del18), E746_T751>A (2237_2251del15) L747_T751del (2239_2253del) L747_T751>P (2239_2251>C), L747_E749del (2239_2247del9), E746_E749del (2235_2246del12), L747_P753>Q (2239_2258>CA), L747_T751>S (2240_2251del), E746_S752>A (2237_2254del18)
L747_A750>P (2238_2248>GC), 9E746_S752>D (2238_2255del18), E746_T751>I (2235_2252>AAT), L747_T751>Q (2238_2252>GCA), E746_T751del (2236_2253del18)