The TRUPCR® MPN Panel Kit is intended for the qualitative detection of JAK2-V617F, CALR, MPL W515K/L mutations and BCR-ABL1 translocation in Myeloproliferative neoplasms (MPNs) in peripheral blood samples using real-time PCR.
Myeloproliferative neoplasms (MPN), previously called myeloproliferative disorders (MPD) are a group of diseases that are caused by an overproduction of one or more blood cell types (red cells, white cells or platelets) in the bone marrow. Myeloproliferative neoplasms (MPNs) are associated with dysregulation of tyrosine kinases, leading to abnormal downstream signaling pathways and increased cellular proliferation. Based on the presence or absence of the Philadelphia chromosome (BCR/ABL1 translocation), MPN are broadly grouped into two categories - Philadelphia positive (chronic myeloid leukemia) and Philadelphia negative (polycythemia vera, essential thrombocythemia and myelofibrosis). The Philadelphia chromosome negative MPNs are characterized by mutations in various genes such as JAK2, MPL, CALR, PDGFRA, PDGFRB, FGFR1 and KIT. In addition, multiple chromosome abnormalities have been defined.
TRUPCR® MPN panel Kit is a Real-time PCR assay which is based on fluorescent signal generated from the presence of an oligonucleotide probe specific for target DNA sequence. TRUPCR® MPN Panel kit is a comprehensive kit which includes complete cDNA chemistry for RNA based transcripts.
e13a2 & e14a2 (p210), e1a2 (p190)
Type 1 and Type 2
W515L and W515K